Is there a way to sequence the entire 16S gene (~1.5kbp) on an Illumina MiSeq?

2022.01.28 06:39 borneatsea Is there a way to sequence the entire 16S gene (~1.5kbp) on an Illumina MiSeq?

Although the largest kit they offer is the v3 (2x300 bp) I came across this paper, and for the life of me I can not figure out how they did it:
(TLDR they sequenced the entire 16S-23S region on a v3 kit, and the only reference to primers/adaptors is during PCR stage)
It was my understanding that because we would want an overlap between your forward and you reverse reads that in practice you would only be able to sequence something of about 550bp, anything higher is risky.
My aim is to choose a method for bacterial IDs that avoids primer biases which are common when only sequencing certain regions within the 16S gene. The only way I could see them getting around the ~550bp limit is by amplifying the entire 16S-23S region, fragmenting it, and then indexing it(??) almost like a WGS approach of the amplicon in its entirety.
Any ideas or theories are welcome :)
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2022.01.28 06:39 MagmaHotDesigns It’s like poetry

It’s like poetry submitted by MagmaHotDesigns to Kanye [link] [comments]

2022.01.28 06:39 Gordons_Donut Can I use egg wash before freezing?

I am preparing some chicken meatballs with a twist, there is a gravy filling in between the meatballs. It’s for a family dinner so I’ll have a lot more to do and I wanted to freeze them a day before the dinner so that I only need to fry them straight out of the freezer. I am using egg wash to bind the bread crumbs onto the meatballs will there be any issue with freezing?
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2022.01.28 06:39 Linasprolt Oi tie nausedos norai

Oi tie nausedos norai submitted by Linasprolt to deMiko [link] [comments]

2022.01.28 06:39 FullSand RYLAIS + HORIZON FOCUS S12 GAMING STREAM

RYLAIS + HORIZON FOCUS S12 GAMING STREAM submitted by FullSand to azirmains [link] [comments]

2022.01.28 06:39 gslondontyf Introducing: First 3.0 Metaverse Gaming NFTs - 29TH JANUARY 15:00 UTC - CERTIK/SOLIDPROOF

Cyberclassic is a futuristic Gaming NFTs-marketplace and issuance protocol that utilizes token utility to empower the cyborg metaverse community.

The NFT not only gives players ownership of the in-game assets they have purchased, but also allows the players to safely trade those assets. An NFT has multiple use cases across different industries. Due to its intrinsic proof of ownership property, these non-fungible tokens are used in gaming, metaverse, social media, Art, and other renowned industries.
With a total NFT supply of 7000 cyborgs, this project aims to bring the future to life. The target community of Cyberclassic are forward looking futurists who’d prefer to have some sort of connection with the robotic future that has been long discussed in fiction. These cyborg NFTs are partial robots with some human-like characteristics. Besides being highly visually appealing, these characters come in both genders and different ethnicities to entertain a vast community. Initially only common characteristics NFTs will be available for auction; with advancements in the project, rare and legendary characteristics will also be unlocked, depending on the response from the community.

Web 3.0 and Metaverse
The Cyberclassic metaverse serves as a gateway to a new virtual world full of opportunities. As of now, our team aims to launch virtual reality for the community, where our community and board members will be able to have virtual meetings and play RPG games.

Cyborg Marketplace
Cyberclaasic marketplace is a bazaar of opportunities; where our users can gather to trade their bionics. Our marketplace moves the market game up another step by offering metaverse and in game assets transfer. Metaverse’s virtual reality component will capacitate users to play competition games and earn rewards. This will enhance user experience by facilitating transactions and providing versatile options.

Cyberclassic staking portal
Cyberclassic aims to have a staking portal that enables utility token investment. Initially only a set of staking pools with locked liquidity presumption will be launched at the portal. These liquidity pools will have a liquidity locking time frame of small/medium/large liquidity cycles with APY ranging from 500% to 1500% Each liquidity pool will possess a certain level of liquidity intake that will be based on the merit of first come first serve basis. Users can also increase their reward through opting for longer liquidity cycles.

Cyberclassic Use Case

Digital collectible
Cyberclassic provides a wide variety of cyborg characters on its platform, that too, in the form of collectible non-fungible tokens. These cyborg NFTs are a view of the future, a future of genome engineering, AI, machine learning and robotics. These collectible NFTs are a sneak peek into our advanced research and development.

Cyberclassic constantly entertains its users through periodic events and tournaments on the platform that invites all users to participate while providing them a chance to earn exciting rewards.

Cyberclassic game is a merger of three gaming models that includes a subsequent type of RPG with a combination of card games and NFT collectibles. The intrinsic model of Cyberclassic games is a play to earn model. The basic agenda of the game is to incentivize game play.

Investor status
Cyberclassic platform offers staking on $CLASS utility tokens in return for upto 1500% APY. Prior to the launch of the game, staking will offer users of the platform to earn exciting rewards as a passive source of income.

DAO governance
Some cyborg NFTs are voting tokens. These NFTs especially enable its holder to participate in all matters of the Cyberclassic platform, from tokenomics, to the launch of new features and NFTs. The vote token holder will be a member of the governance society where all members gather to reach a mutual consensus for the advancement of the platform.

Balancing via governance
Balance patches can be executed much more dynamically than traditional matches due to the data-driven nature of the underlying Cyberclassic codebase. Each patch will be a Neural Net Assisted update that is voted on by the council and takes into account the thousands of expected matches in the game.

➔Staking: 36%
➔P0 – 10%
➔P1 – 10%
➔P2 – 4%
➔P3 – 4%
➔P4 – 4%
➔P5 – 4%
➔Presale: 30%
➔Liquidity: 16.5%
➔Private seed funds: 5%
➔Treasury: 3%
➔Marketing/Airdrop: 3%
➔Team/Advisory: 2%
➔ILO Fee: 2%
➔Contingency: 1.5%
➔Ecosystem: 1%

Cyberclassic Token Utility
Cyberclassic has its own utility token $CLASS that is used as a native currency on the platform and its game. Players are rewarded with $CLASS tokens to win battles, and tournaments. The intrinsic property of the utility token is demand driven, that increases its value based on the market demand. $CLASS token value is determined through the revenue generating model of the project. Moreover, the project team is testing on a PVE game to entertain the community.

Cyberclassic Roadmap

Q4 2021
❖Key advisors commence development
❖Website creation
❖Whitepaper release
❖Social media presence
❖Smart contract audit
❖Press release / Yahoo/Benzinga/Financial Post/Marketwatch
❖Community reward/Giveaway
❖Certik Audit/Solidproof Audit
❖Strategic Partnerships

Q1 2022
❖5% initial sale to exlusive VC’s/investors
❖Staking platform launch
❖NFT minting
❖Launch on pancakeswap
❖Key influencers onboarding / AMA
❖Coin market cap and coin market listing
❖CEX listing
❖Strategic partnership with key partners and leading brands
❖RPG metaverse game on testnet
❖Governance DAO
❖Gaming Tournaments
❖Coingecko/Coinmarketcap listing
❖Deploy Contracts on Polygon blockchain
❖Collaboration & Partnership
❖NFTs Airdrop for presale participants

Q2 2022
❖Mobile app development
❖Launch of test net game for cross chain
❖Live NFTs marketplace on testnet
❖Deploying of Cyberclassic NFT platform on main net
❖NFTs Mint for 3rd round
❖Mobile Application
❖RPG metaverse game on mainnet
❖P2E Game on Testnet
❖VIP Membership

Q3 2022
❖Community attestation
❖NFTs Mint for 3rd round
❖Reward on staking NFT platform
❖Media collaboration
❖Research and development
❖Unlocking lottery system (Lottery event participation that rewards Cyberclassic native token)
❖P2E Game on Mainnet

Vision and Mission
Our mission and vision is to revolutionize blockchain gaming while keeping the true essence of card games alive. Cyberclassic game is inspired by our childhood favorite pokemon cards. It is an attempt to bring new gaming technologies in our old games to strike the right balance of innovation and nostalgia. We aim to transform Cyberclassic into a metaverse ecosystem. This platform is not limited to NFT and tokens. We aim to provide entertainment and a fun way to learn and earn in crypto space. Cyberclassic is the future of NFTs.

submitted by gslondontyf to CRYPTOPUMPCLUB [link] [comments]

2022.01.28 06:39 ThrowRA_0012234c2c Is it wrong of me to want to break up?

TW: Self harming, suicide, depression.
Hey all, my girlfriend and I are in our early 20s. We met around 8 months ago and have been together since a few months.
For that period I've had a great time where we would hang out 24/7 playing games, studying together, talking etc.
We wanted to move in together in the next few years (either her with me or me with her).
On the other hand there have been some issues. She frequently has thoughts of suicide and also self harms frequently... I didn't know to this often extent, basically whenever she is sad. Over the last few months I'd do everything I can to be there for her and very frequently give up what I wanted to do that night just go be there for her. I sacrificed friendships and hobbies to try and prevent her from doing anything bad. In my previous posts I was exploring whether this dependency is bad and I established it is, as I'm not her therapist.
I myself have been diagnosed with severe depression, and it's coming to a point where I don't have the energy to be there for her whist I'm also trying to rekindle the fire of other friendships and hobbies. It has gotten to the point recently where I'm struggling to feel anything like care/worry instead only stress when she talks so negatively. I also notice that me being there for her is also bringing down my own mental health. I realize I'm not ready for a relationship at the moment and in addition I'm questioning if she is the right person for me. I know mental health issues are like a "disease" that can be cured but my gut tells me she's not the one. But I'm scared my gut is talking nonsense.... Anyone had a similar issue??
submitted by ThrowRA_0012234c2c to LongDistance [link] [comments]

2022.01.28 06:39 Top_Feedback_4773 Cavalry of the Korean Empire in 1903

Cavalry of the Korean Empire in 1903 submitted by Top_Feedback_4773 to MilitaryHistory [link] [comments]

2022.01.28 06:39 embiate Big E or something? Idk I don't watch WWE anymore.

Big E or something? Idk I don't watch WWE anymore. submitted by embiate to Wrasslin [link] [comments]

2022.01.28 06:39 CapableGTRRRRAH 'What will they send next? Pillows?': Kyiv mayor Vitali Klitschko hits back at Berlin over helmets

'What will they send next? Pillows?': Kyiv mayor Vitali Klitschko hits back at Berlin over helmets submitted by CapableGTRRRRAH to europe [link] [comments]

2022.01.28 06:39 Lolis4TheWin My first laptop, my first thinkpad and my first linux machine

First of all sorry for my bad English. This is my first “real” post.
I was looking for a laptop because I'm going next year to universiti (if everything will as planed). I hate when I have an enormous variety of things I want to buy for example phones, shoes etc. I alway try to reduce it to a certain model or a manufacturer, in this case the Thinkpad series.
So about a half month ago i bought my first laptop. It is a T480. It was about 450 euros which i consider a really great deal.
It contains: - i5-8350u - 8gb of ram - 256 nvme ssd - 2x24 w battery - 1080p display
When i first saw and touched the machine i was shocked. This build quality is awesome! (Although the cover is a fingerprint magnet…)
I wasnt expressed by the keyboard at first, i mean i havent felt that “the best laptop keyboard” feeling, but after a week of use i think i can understand why do peaple say that. I came from a 100% keyboard( keyboard with numpad) , so the placing (?) of the “home”, “end”, “prtsc” and the “fn” keys are weird, but i think i can will get use(d) to it…
The trackpad. Well… I dont really like it. I mean the trackpoint is a good thing, but i dont think i will use it as much as it intended (but i really like it). I mean it is alright or its just weird cause of me who only used mouse his entiry life.
I also dont like, or i could say hate the power button. Its horrible, but im thankful that is the main thing i have about this laptop.
I really miss the “pause, next, previous” media control keys. (I managed to imitate it via shortcuts.)
When i first got it it had a win10 and everything was swedish, i “reinstalled” it, but still havent figured out the full language change, but that dosent matter.
I made a bios update and then headed to my Manjaro xfce boot drive.
I installed manjaro with open source drivers and everything critical worked out of the box (wifi, touchpad etc.)
Im “completely” new to linux, so i was really glad.
I had to “configure” two necessary things: TLP and Gestures. In aur you can find gui for these tool, so its kinda easy.
With linux i have a freaking great experience. Everyone said 8gb or ram is not enough, but i have 10+ page in Firefox, spotify, caprine, pycharm running and it is about 4gb usage. Amazing.
Conclusion. I wont regret this. I thing this laptop was one of my best deal in my life.
submitted by Lolis4TheWin to thinkpad [link] [comments]

2022.01.28 06:39 Large-Maintenance-30 What to look for when buying TSDZ2 ?

I'm gonna buy a tsdz2 but there are several models (or controllers ?) and I don't know what to choose... Say I buy a 36v350w version, is the unit different from the 48v750w or is it just the controller settings ? I'd like to tinker with the settings so I'm gonna use the open source firmware, does this affect the display I need to get ? I would also love to have a throttle What to look out for when buying to get what I need ?
submitted by Large-Maintenance-30 to ebikes [link] [comments]

2022.01.28 06:39 peachyheart444 Broke no contact after 2 weeks..

He texted me that he wanted to come get the rest of his stuff. I said that was fine and when he got here he started crying and telling me how much he loved me and how much he cared about me and misses me. I held my ground at first but I ended up crying with him and we talked for a couple hours. We were hugging and holding each other.
After he left I felt so set back in healing. It felt SO good to be around him but he is not good for me. I feel way sadder than before. I feel like a drug addict, I just keep thinking about seeing him 1 more time. I want to believe his beautiful words but I have to remember his horrible actions in the relationship..
submitted by peachyheart444 to ExNoContact [link] [comments]

2022.01.28 06:39 Eclectix1 Seamaster stopped working

My almost "a decade and a half old" Seamaster Chrono just stopped working today. The stopwatch function also does not work and no movement on the 9 'o Clock subdial either. Wound it 50 times as well as shaking it, but to no avail and I've never had any issues with it.
I remember dropping it just once a few years ago and no great knocks but I've haven't gotten it serviced in all these years. I can hear the sound of the rotor winding.
Anything I can do before I take it to an AD ? Any advice would be much appreciated. Thank you.
submitted by Eclectix1 to OmegaWatches [link] [comments]

2022.01.28 06:39 axbeard Sources on claims in FAQ?

I'm unable to find sources for some of the claims made in the sidebar. Is there a place where all of this stuff is listed? The one that I'm specifically interested in is the one mentioning the suppression of the immune system during fasting, and haven't yet been able to find information on that googling or in the FAQ.
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2022.01.28 06:39 EstablishmentFar6607 me_irl

me_irl submitted by EstablishmentFar6607 to me_irl [link] [comments]

2022.01.28 06:39 Agreeable_Double_237 How non-Arabs understand Juma speech in Mecca?

submitted by Agreeable_Double_237 to AskMiddleEast [link] [comments]

2022.01.28 06:39 Ok_Difference_4045 Medical School interview at Bialystok in 5 days [Nervous] [Competitive]

How do I calm down? It's very competitive and I'm not sure I will get in..

I need 51/100 points in the interview to get in, and they only have 140 places.

It's in Poland so it's quite competitive.

They will interview people from 12-15, so I have to potentially wait 3 hours.. Welp!

Thanks in advance guys..
submitted by Ok_Difference_4045 to medicalschoolEU [link] [comments]

2022.01.28 06:39 Ishy_BonsaiTree Besties

Besties submitted by Ishy_BonsaiTree to CatsWithDogs [link] [comments]

2022.01.28 06:39 DrunkBamboo Help I want to fap so bad

I really want to change, I want to change. But at this moment, none of my friends are responding to any of my messages and I'm tired of playing videogames. I don't feel like doing anything at the moment so I'm back to fapping but I stopped. I don't want to. The urge is killing me, help district please.
submitted by DrunkBamboo to NoFap [link] [comments]

2022.01.28 06:39 Great-Obligation-599 Bugs and Sylvester sitting on thrones

Bugs and Sylvester sitting on thrones submitted by Great-Obligation-599 to looneytunes [link] [comments]

2022.01.28 06:39 muffingaming77 Will you sign my petition?

Petition for there to be a series similar to Red Vs. Blue but Destiny.
submitted by muffingaming77 to destiny2 [link] [comments]

2022.01.28 06:39 Clondom_ I am thinking to do another concept card for Mk Mobile. I want you to choose between these options.

If you have other ideas write in the comments!
View Poll
submitted by Clondom_ to StarInSkyClub [link] [comments]

2022.01.28 06:39 Dan11106 Aeotec Multisensor 7

For this interested Aeotec has an online store now. Maybe some knew but I didn't.
submitted by Dan11106 to homeassistant [link] [comments]

2022.01.28 06:39 LISETTE-ARTIST Artwork from 2017.

Artwork from 2017. submitted by LISETTE-ARTIST to CreepyArt [link] [comments]